| Type |
Conjugated Primary Antibody |
| Conjugated with |
ALEXA FLUOR® 594 |
| Host organism |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide |
FOXG1/BF-1 |
| Specificity |
This antibody reacts specifically with FOXG1/BF-1 |
| Modification |
No modification has been applied to this antibody |
| Modification site |
None |
| Clonality |
Polyclonal Antibody |
| Clone |
Polyclonal Antibodies |
| Concentration |
1ug per 1ul |
| Subcellular locations |
N/A |
| Antigen Source |
KLH conjugated synthetic peptide derived from human FOXG1 |
| Gene ID |
N/A |
| Swiss Prot |
N/A |
| Applications |
IF(IHC-P) |
| Applications with corresponding dilutions |
IF(IHC-P)(1:50-200) |
| Cross reactive species |
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus) |
| Cross Reactive Species details |
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species. |
| Background information |
The winged-helix transcriptional repressor (WH) BF-1 gene encodes brain factor 1 (BF-1), also known as foxg1, and is essential for the proliferation of progenitor cells in the cerebral cortex and influences regional patterning in the mammalian telencephalon (1–4). WH proteins are a family of putative transcriptional regulators with diverse roles in development, and are characterized by a highly conserved DNA binding structure, the WH domain (1,5,6). BF-1 plays a critical role in the development of the cerebral hemispheres of the brain and targeted disruption of the gene leads to severe defects in the development of telencephalic structures, such as the cerebral cortex and basal ganglia (1). The loss of BF-1 results in an accelerated rate of neuronal differentiation and the shortening of the neurogenetic period in the embryonic cerebral cortex (1,7). BF-1 is expressed by E8.5 in telencephalic progenitors (1). It may also regulate the response of cerebral cortical progenitors to environmental cues (1). |
| Purification method |
Purified by Protein A. |
| Storage |
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months. |
| Excitation emission |
590nm/617nm |
| Synonyms |
BF 1; BF 2; BF-1; BF-2; BF1; BF2 ; Brain factor 1; Brain factor 2; FHKL; FKH2; FKHL1; FKHL2; FKHL2; FKHL3; FKHL4; Forkhead box G1A; Forkhead box G1B; Forkhead box protein G1; Forkhead box protein G1A; Forkhead box protein G1B; Forkhead box protein G1C; Forkhead drosophila homolog like 2; Forkhead like 1; Forkhead like 2; FOXG1_HUMAN. |
| Also known as |
FOXG1/BF-1 Polyclonal Antibody |
| Other name |
Anti-FOXG1/BF-1 Polyclonal |
| Advisory |
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom. |
| Properties |
For facs or microscopy Alexa 1 conjugate. |
| Conjugation |
Alexa Fluor,ALEXA FLUOR® 594 |
| Conjugated |
Alexa conjugate 1 |
| Description |
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. |
| Group |
Polyclonals and antibodies |
| About |
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. |